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cidar moclo extension  (Addgene inc)
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Addgene inc cidar moclo extension
A . Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a <t>CIDAR</t> cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. B . Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from Supplementary Figure 1. Expression from the original vector series in E . coli matches closely to the expression reported in this study. C . Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. D . The same plots in as in C for the same constructs expressed in P. putida . E . The same plots in as in C for the same constructs expressed in C. necator . F . The same plots in as in C for the same constructs expressed in K. nataicola .
Cidar Moclo Extension, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A . Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a CIDAR cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. B . Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from Supplementary Figure 1. Expression from the original vector series in E . coli matches closely to the expression reported in this study. C . Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. D . The same plots in as in C for the same constructs expressed in P. putida . E . The same plots in as in C for the same constructs expressed in C. necator . F . The same plots in as in C for the same constructs expressed in K. nataicola .

Journal: bioRxiv

Article Title: Systematic part transfer by extending a modular toolkit to diverse bacteria

doi: 10.1101/2023.02.07.527528

Figure Lengend Snippet: A . Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a CIDAR cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. B . Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from Supplementary Figure 1. Expression from the original vector series in E . coli matches closely to the expression reported in this study. C . Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. D . The same plots in as in C for the same constructs expressed in P. putida . E . The same plots in as in C for the same constructs expressed in C. necator . F . The same plots in as in C for the same constructs expressed in K. nataicola .

Article Snippet: The CIDAR MoClo Parts Kit (#1000000059) and CIDAR MoClo Extension, Volume I (#1000000161) were purchased from Addgene.

Techniques: Cloning, Expressing, Plasmid Preparation, Construct